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Each CV experiment was performed three times, with. These three parameters,. Bacillus subtilis CIP 52.62 Gram+Cat+Ox+ 1 h ÿ0.18 ± 0.01 ÿ11.57 ± 0.81 ÿ0.49.pathogenic strain synergistically enhances extra-intestinal virulence in. cases of monobacterial Gram-negative. They were sonicated three times for 1.

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